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KMID : 0613820060160040676
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Journal of Life Science
2006 Volume.16 No. 4 p.676 ~ p.682
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Characteristics of Insoluble Phosphates Solubilizing by Klebsiella sp. DA71-1/pLYJ
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Ryu Ah-Reum
Lee Jin-Woo
Lee Yong-Seok
Lee Sang-Cheol
Jung Soo-Yeol
Choi Yong-Lark
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Abstract
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To develop high efficiency biofertilizer solubilizing insoluble phosphates, lactate dehydrogenase (ldh) gene was isolated from Staphylococcus sp. LJ2. Genetic constructions were carried out using the pGEM-T-easy vector and pHSG398. Recombinant DNA plasmids containing the ldh gene were transferred to Klebsiella sp. DA71-1 by electroporation. The selected transformant was named as a DA71-1/pLYJ. The insoluble phosphates solubilization activity of DA71-1/pLYJ was higher than that of DA71-1 at various culture conditions. Glucose was the best carbon source for insoluble phosphates solubilization among the used carbon sources. Maximal insoluble phosphates solubilizing was found in sucrose minimal (SM) medium containing 3% glucose. The solubilizing activity of DA71-1/pLYJ against three types of insoluble phosphates, such as tri-calcium phosphate, hydroxyapatite, aluminium phosphate, were quantitatively determined. The optimal temperature and initial pH to solubilize insoluble phosphates in the SM medium was 37℃ and pH 5.0, respectively.
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KEYWORD
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Biofertilizer, lactate dehydrogenase, insoluble phosphates, sucrose minimal medium, Klebsiella sp
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